1381289-58-2

Ribocil is a synthetic mimic of riboflavin 5’-monophosphate (FMN; ) that competes with the natural ligand to inhibit FMN riboswitch-mediated expression of ribB, a gene responsible for the synthesis and transport of riboflavin. Ribocil depletes cellular levels of riboflavin with an IC₅₀ value of 0.3 μM and inhibits E. coli growth with an MIC value of 2 μg/ml.

Ribocil is a highly selective chemical modulator of bacterial riboflavin riboswitches. Ribocil strongly inhibits GFP expression, achieving a 50% effective concentration (EC50) of 0.3 μM. Target: in vitro: Ribocil is a highly specific bioactive synthetic mimic of FMN, which competes with the natural ligand to inhibit FMN riboswitch-mediated expression of ribB and inhibits bacterial growth. Ribocil-B demonstrates superior microbiological activity as compared to Ribocil-A (minimum inhibitory concentration (MIC) = 1 μg/ml versus MIC ≥ 64 μg/ml), inhibition of riboflavin synthesis (IC50 = 0.13 μM versus IC50 > 26 μM), and binding affinity to the E. coli FMN aptamer (Kd = 6.6 nM versus Kd ≥ 10,000 nM).[1]

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[1] Howe JA, et al. Selective small-molecule inhibition of an RNA structural element. Nature. 2015 Oct 29;526(7575):672-7. DOI:10.1038/nature15542