Description
Z-
VAD(OH)-
FMK is an irreversible tripeptide inhibitor of all caspases. This non-
methylated form is useful in studies involving recombinant, isolated, or purified enzymes. Unlike the methylated form, Z-
VAD(OMe)-
FMK , this compound does not require pretreatment with esterases prior to
in vitro use.
Uses
Z-VAD-FMK (Z-VAD(OH)-FMK) is a well-know pan caspase inhibitor, which does not inhibit ubiquitin carboxy-terminal hydrolase L1 (UCHL1) activity even at concentrations as high as 440 μM[1].
General Description
An irreversible general caspase inhibitor. Useful for studies involving recombinant, isolated, and purified caspase enzymes. Unlike Caspase Inhibitor I (Cat. No.
627610), this inhibitor does not require pretreatment with esterase for
in vitro studies. A 10 mM (1 mg/221 μl) solution of Caspase Inhibitor VI (Cat. No.
219011) in DMSO is also available.
Biochem/physiol Actions
Product does not compete with ATP.
References
[1] Davies CW, et al. The co-crystal structure of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) with a tripeptide fluoromethyl ketone (Z-VAE(OMe)-FMK). Bioorg Med Chem Lett. 2012 Jun 15;22(12):3900-4. DOI:
10.1016/j.bmcl.2012.04.124[2] Liu HR, et al. Antiproliferative activity of the total saponin of Solanum lyratum Thunb in Hela cells by inducing apoptosis. Pharmazie. 2008 Nov;63(11):836-42. PMID:19069247
