Description
Arachidonoyl thio-PC is a substrate for many phospholipase A
2s (PLA
2s) including sPLA
2, cPLA
2, and iPLA
2. Cleavage of the
sn-
2 fatty acid by PLA
2 results in generation of a free thiol which reacts with chromogenic reagents such as DTNB (Ellman’s reagent) and DTP to allow quantitation of PLA
2 activity. Isozyme-
specific cPLA
2 activity can be measured by excluding or inhibiting sPLA
2 and iPLA
2 activities in the assay.
Uses
Arachidonoyl Thio-PC is a substrate of many phospholipase A2 (PLA2), including sPLA2, cPLA2 and iPLA2. Cleavage of sn-2 fatty acids by PLA2 results in the production of free thiols, which react with chromogenic reagents such as DTNB (Ellman's reagent) and DTP, allowing quantification of PLA2 activity. Isozyme-specific cPLA2 activity can be measured by depleting or inhibiting sPLA2 and iPLA2 activity in the assay.
