1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-6,8-disulfo-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-6,8-disulfo-, inner salt, potassium salt (1:3)

Product Name1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-6,8-disulfo-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-6,8-disulfo-, inner salt, potassium salt (1:3)
CAS2419286-92-1
MFC44H46KN3O16S4
MW1040.2
EINECS
MOL File2419286-92-1.mol

Chemical Properties

storage temp.	Store at -20°C, away from moisture
solubility	Easily soluble in organic solvents such as DMSO and DMF, very soluble in water
form	Solid
color	Blue to dark blue
Appearance	Dark blue solid
ex/em	678/706 nm (PBS buffer)
ε(extinction coefficient)	211000 L⋅mol−1⋅cm−1
Φ(quantum yield)	0.21

Usage And Synthesis

Description

sulfo-Cyanine5.5 NHS ester is an amine-reactive activated ester of sulfonated far-red Cyanine5.5 fluorophore, an analog of Cy5.5®. This is a reagent of choice for the labeling of antibodies, sensitive proteins, and others which require reactions in a purely aqueous environment or without any significant additions of organic co-solvents.

The dye is well-suitable for non-invasive in vivo NIR imaging and applications requiring low fluorescent background.

Chemical Properties

Appearance: Dark blue solid
ex/em: 678/706 nm (PBS buffer)
Extinction coefficient (ε): 211000 Lmol1cm1
Quantum yield (Φ): 0.21

Uses

Sulfo-CY-5.5 NHS ester (Sulfo-Cyanine5.5 NHS ester) tripotassium is an amine-reactive ester of sulfonated far-red Cyanine5.5 fluorophore, which can be used to label antibodies, proteins, etc., as well as for in vivo NIR imaging[1].

References

[1] Xiaoyu Xu, et al. Microfluidic-assisted biomineralization of CRISPR/Cas9 in near-infrared responsive metal-organic frameworks for programmable gene-editing. Nanoscale. 2022 Nov 3;14(42):15832-15844. DOI:10.1039/d2nr04095f

Abs/Em Maxima

675/694 nm

Fluoresene quantum yield

0.21

Extinction Coefficient

211000

CF260

0.09

CF280

0.11

1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-6,8-disulfo-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-6,8-disulfo-, inner salt, potassium salt (1:3) Supplier

1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-6,8-disulfo-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-6,8-disulfo-, inner salt, potassium salt (1:3) manufacturers

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