SYBR(R) GREEN I NUCLEIC ACID GEL STAIN

Product NameSYBR(R) GREEN I NUCLEIC ACID GEL STAIN
CAS163795-75-3
MFC32H37N4S
MW0
EINECS999-999-2
MOL FileMol File

Chemical Properties

Flash point	94℃
storage temp.	-20°C
solubility	good in DMSO
form	solution
Appearance	orange crystals

Safety Information

WGK Germany	1
F	3
Storage Class	10 - Combustible liquids

MSDS

Provider	Language
SigmaAldrich	English

Usage And Synthesis

Description

dsGreen® is a highly sensitive and selective stain for double-stranded DNA (dsDNA). dsGreen solutions are used for DNA binding and gel staining. This product is a solid form of dsGreen that can be used to prepare solutions according to research protocols. Lumiprobe also manufactures dsGreen in the form of a solution specifically optimized for real-time PCR.

Uses

SYBR Green I nucleic acid gel stain has been used for real-time PCR quantification and flow cytometry.

Definition

ChEBI: SYBR Green I is a cationic unsymmetrical cyanine dye used as a nucleic acid stain in molecular biology. SYBR Green I binds to double-stranded DNA.

General Description

SYBR Green I is an asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

Abs/Em Maxima

454/524 nm