Janelia Fluor 549, free acid

Janelia Fluor 549 TFA (JF549 TFA) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

Cell-permeable, yellow fluorescent dye supplied as free acid. Used for the synthesis of Janelia Fluor HaloTag and SNAP-Tag ligands for use in live cell imaging experiments (Grimm et al 2017) . Also suitable for flow cytometry. Janelia Fluor 549 is 2 x brighter than TMR and Cy3in vitroand live-cell experiments.Spectrally similar dyes:Alexa Fluor 546, Alexa Fluor 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

-20°C

[1] Jonathan B Grimm, et al. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol Biol. 2017;1663:179-188. DOI:10.1007/978-1-4939-7265-4_15