RI-1

RI-1 (415713-60-9) is a potent and selective inhibitor of RAD51 (IC50 = 5-30 μM), a highly conserved protein that catalyzes DNA repair via homologous recombination.1 It specifically reduces gene conversion in human cells and stimulates single strand annealing. It covalently binds to Cys319 on the surface of RAD51 and disrupts protein-protein interaction. It potentiates the effect of DNA-damaging agents on tumor cells and is a novel tool for studying DNA repair in cells.2 Potentiates the killing of glioblastoma cells by ionizing radiation3 and alkylating drugs4.

RI 1 is a chemical inhibitor of RAD51, which disrupts homologous recombination in human cells.

RI-1 is a cell-permeable RAD51 inhibitor that binds covalently to the protein surface at Cysteine 319. RI-1 disrupts homologous recombination in human cells.

RAD51

[1] RANJITH ANAND. Rad51-mediated double-strand break repair and mismatch correction of divergent substrates[J]. Nature, 2017, 544 7650: 377-380. DOI:10.1038/nature22046
[2] BRIAN BUDKE. RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells.[J]. Nucleic Acids Research, 2012, 40 15: 7347-7357. DOI:10.1093/nar/gks353
[3] ANAÏS BALBOUS. A radiosensitizing effect of RAD51 inhibition in glioblastoma stem-like cells.[J]. BMC Cancer, 2016: 604. DOI:10.1186/s12885-016-2647-9
[4] NANCY BERTE. Targeting Homologous Recombination by Pharmacological Inhibitors Enhances the Killing Response of Glioblastoma Cells Treated with Alkylating Drugs.[J]. Molecular Cancer Therapeutics, 2016, 15 11: 2665-2678. DOI:10.1158/1535-7163.mct-16-0176