N-terminal HIS-tagged fusion protein corresponding to full-length Green Fluorescent Protein, expressed in E. coli. Green fluorescent protein (GFP) is a cytoplasmic protein found in jellyfish Aequorea Victoria.
Green fluorescent protein (GFP) is genetically fused to many proteins in various species to generate non-reactive chimeras which are known to preserve their original biological activity as well as the fluorescent properties of native GFP. GFP acts as a reporter for gene expression and as a fusion tag for protein localization studies in live cells. It serves as a spectroscopic and microscopic probe to understand the biological functions of various systems. GFP is highly stable and can serve as a tool for in vitro studies.
rGFP Aequorea victoria produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-238 a.a.) and having a molecular mass of 26.8 kDa.
rGFP is purified by proprietary chromatographic techniques.
GFP is an intermediate filament. GFP and vimentin are linked to the same filament network; they are localized in the same filaments.
mRNAs encoding the glial intermediate filament protein are spatially dispersed in the glial cell cytoplasm close to the location of the glial filaments.
