ANTI-AKT1抗体

兔多克隆抗体to AKT1

Rabbit

与反应: Human
预测可用于: Mouse, Rat, Cow, Xenopus laevis

Western blot - Anti-AKT1 antibody (ab235958)

All lanes : Anti-AKT1 antibody (ab235958) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

Anti-AKT1抗体是一类可以特异性结合Anti-AKT1的多克隆抗体，主要用于检测Anti-AKT1的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。

双抗体夹心法测定标本中Anti-AKT1水平。用纯化的Anti-AKT1抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Anti-AKT1，再与HRP标记的Anti-AKT1抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Anti-AKT1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中Anti-AKT1浓度。

ANTI-AKT1抗体用于双荧光素酶报告基因检测miR-185与AKT1靶标关系研究