| Name | 1-EBIO |
| Description | 1-EBIO (1-EBIO) is a calium channel agonist. |
| Kinase Assay | Procedures for BTK OMNIA Assay: The Omnia continuous read assay is performed essentially as described by the vendor. The assay conditions are: 40 μM ATP (1X KMATP), 10 μM Y5-Sox, and 10 nM BTK enzyme. Briefly, a substrate mix containing 1.13X ATP and the Y5 Sox substrate is first prepared in 1X Omnia Kinase Reaction Buffer (KRB) consisting of 20 mM Tris, pH 7.5, 5 mM MgCl2, 1 mM EGTA, 5 mM?β-glycerophosphate, 5% glycerol, and 0.2 mM DTT. For IC50 measurements, 5 μL of enzyme are incubated with serially diluted (3-fold) compounds prepared in 50% DMSO in a Corning (#3574) 384-well, white, non-binding surface microtiter plate at 25°C for 30 min. Kinase reactions are started with the addition of 45 μL of the ATP/Y5 substrate mix and monitored at λex360/λem485 in a Synergy 4 plate reader for 60 minutes. Progress curves from each well are examined for linear reaction kinetics and fit statistics. Initial velocity from each reaction is determined from the slope of a plot of relative fluorescence units versus time and then plotted against inhibitor concentration to estimate IC50 using the Response, Variable Slope model in GraphPad Prism from GraphPad Software. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (12.33 mM), Sonication is recommended.
DMSO : 60 mg/mL (369.94 mM), Sonication is recommended.
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| Keywords | Potassium Channel | KcsA | Inhibitor | inhibit | CalciumChannel | Calcium Channel | 1Ethyl2benzimidazolinone | 1 Ethyl 2 benzimidazolinone |
| Inhibitors Related | Quadrol | 2,4,6-Tri-tert-butylphenol | Chlorocresol | L-Ascorbic acid | 1,8-Cineole | Tetraethylammonium bromide | Chenodeoxycholic acid | L-Ascorbic acid sodium salt | 2-Nitrobenzoic acid | Chlorzoxazone | Magnesium Chloride Hexahydrate | Magnesium sulfate |
| Related Compound Libraries | Pain-Related Compound Library | Bioactive Compound Library | Neuronal Signaling Compound Library | Membrane Protein-targeted Compound Library | Neuroprotective Compound Library | Anti-Cardiovascular Disease Compound Library | NO PAINS Compound Library | Metabolism Compound Library | Bioactive Compounds Library Max | Ion Channel Targeted Library | Anti-Cancer Compound Library | Anti-Hypertension Compound Library |
United States
