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ChemicalBook Product Catalog Chemical Reagents Organic reagents Aromatic acids 2-(4-Hydroxyphenylazo)benzoicacid

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2-(4-Hydroxyphenylazo)benzoicacid NEW

$ 50.00 /1g

Min. Order
Purity99.21%
Cas No1634-82-8
Supply Ability10g
Update time2026-04-14

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

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Chemical Properties

Product Name	2-(4-Hydroxyphenylazo)benzoicacid
CAS No	1634-82-8
EC-No
Min. Order
Purity	99.21%
Supply Ability	10g
Release date	2026/04/14

Product Introduction

Bioactivity

Name	2-(4-Hydroxyphenylazo)benzoicacid
Description	2-(4-Hydroxyphenylazo)benzoicacid is a spectrophotometric probe that shows changes in absorption spectra upon binding to proteins and binds to bovine serum proteins.
In vitro	Instructions 1. Storage solution preparation: When preparing the stock solution, deionized water or an appropriate amount of ethanol can be used, and the concentration range is usually 1–10 mM. 2. Operation steps: Binding experiment: 1. Binding of protein and probe: 1) Dissolve the target protein (such as bovine serum albumin, BSA) in an appropriate buffer (such as PBS buffer at pH 7.4). 2) Add 2-(4-Hydroxyphenylazo)benzoic Acid in a certain proportion to ensure that its final concentration is suitable for absorption spectrum measurement (such as 10–50 µM). 2. Incubation conditions: Incubate the mixed system at room temperature or 37°C for 10–30 minutes to ensure that the probe is fully bound to the protein. 3. Absorption spectrum detection: Use a spectrophotometer to scan the absorption spectrum changes in the range of 350–500 nm. After the probe binds to the protein, its characteristic absorption peak (usually around 400 nm) will change significantly. 4. Data analysis: Compare the absorption spectra of the free probe after binding to the protein to determine the binding kinetic parameters. Use titration experiments (such as gradually increasing the probe or protein concentration) to draw binding curves and calculate the binding constant. Notes: 1) The probe is sensitive to pH and a suitable buffer should be used to stabilize the experimental conditions. 2) Prevent light from causing compound degradation or protein denaturation. 3) Before the measurement, ensure that the solution is free of bubbles and particles to avoid interfering with spectral detection. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	Ethanol : 18 mg/mL (74.31 mM), Sonication is recommended.
Keywords	Dye reagent

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