| Name | 2-Di-1-ASP |
| Description | 2-Di-1-ASP is a mono-stryryl dye and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. It has selective for G-quadruplex (G4) and double-stranded DNA. |
| Cell Research | 2-Di-1-ASP as an in situ mitochondrial stain
Procedure
1. Cultivate cells to a suitable state, seed them on a 24 mm diameter coverslip at a pre-confluent density, add 3 μM 2-Di-1-ASP and incubate at 28°C for 40 minutes.
2. Rinse with Hank's balanced salt solution (HBSS) to remove extracellular 2-Di-1-ASP, and then observe mitochondrial activity under a microscope.
The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| In vitro | Compound 18a (2-Di-1-ASP) exhibits marked fluorescence intensification, achieving up to a 300-fold increase, specifically in the presence of G4 structures, while maintaining significant selectivity against double-stranded DNA. Moreover, 2-Di-1-ASP demonstrates fluorimetric selectivity for parallel forms of G4-DNA, including c-kit2, c-myc, and c-kit87up. |
| Storage | Keep away from direct sunlight | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween-80+45% Saline : 2 mg/mL (5.46 mM), Sonication is recommended.
DMSO : 20 mg/mL (54.61 mM), Sonication is recommended.
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| Keywords | probes | mono-stryryl | mitochondrial | Inhibitor | inhibit | G-quadruplex | G4-DNA | Fluorescent | dye | 2-Di-1-ASP | 2Di1ASP | 2 Di 1 ASP |
| Inhibitors Related | 5-Fluorouracil | Adenine hemisulfate | Erythromycin thiocyanate | Guanidine hydrochloride | Hexane-1,6-diol | 1,4-Naphthoquinone | Adenine | Vidarabine | Carbazole | Thymidine | Docosanoic acid | Usnic Acid |
United States
