3-(3-Cholamidopropyl)-dimethylamino-1-propanesulfonic acid (CHAPS) NEW

Purity: 98%

Alias: CHAPS;3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonatehydrate;

3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, zwitterionic; 

Cholamidopropyl-dimethylammonio]-1-propanesulfonate

Biochemical Mechanism: 

CHAPS is a zwitterionic, non-denaturing detergent used for the solubilization of membrane proteins.

Storage Temperature: Store at 2–8°C under a nitrogen atmosphere.

Shipping Conditions: Shipped with ice packs.

Features

CHAPS is a non-denaturing, zwitterionic detergent derived from cholic acid. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.

A non-denaturing, zwitterionic detergent for membrane proteins

Applications

Buffer; zwitterionic nondenaturing detergent for solubilizing membrane proteins CHAPS is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.