3-Morpholinepropanesulfonic acid (MOPS)

3-Morpholinepropanesulfonic acid (MOPS)

Melting Point: 277–282°C

Density: 1.3168 (rough estimate)

Vapor Pressure: 0 Pa at 25°C

Refractive Index: 1.6370 (estimate)

Flash Point: 116°C

Storage Conditions: Store at 25°C, protected from light.

Solubility: H₂O: 1 M at 20°C, clear

Form: Powder/Solid

Color: White

Odor: Odorless

pH Value: 2.5–4.0 (25°C, 1 M in H₂O)

pH Indicator Color Change Range: 6.5–7.9

Acidity Constant (pKa): 7.2 (at 25°C)

Water Solubility: 1000 g/L (20°C)

Maximum Wavelength (λmax): λ: 260 nm, Amax: 0.020; λ: 280 nm, Amax: 0.015; Merck: 14, 6265; BRN: 1106776

Stability: Stable. Incompatible with strong bases and strong oxidizing agents. InChIKey: DVLFYONBTKHTER-UHFFFAOYSA-N; Log P: -2.94 at 20°C

Key Properties:

1. Efficient Buffer: Excellent buffering range of pH 6.5 – 7.9.

2. Low Interference: Exhibits minimal reactivity with metal ions, making it ideal for studies requiring non-coordinating conditions.

3. High Purity: Available in various grades, including cell culture and bio-performance certified.

4. Limitations: It can interfere with the Folin protein assay and partially decompose if autoclaved with glucose.

Applications

1. Cell Culture: Maintains pH stability in culture media for mammalian cells, bacteria, yeast, and other cell types; typically used at concentrations not exceeding 20 mM.

2. Nucleic Acid and Protein Research: Applications include RNA denaturing agarose gel electrophoresis, protein purification, and electrophoretic separation; serves as a commonly used buffering system for RNA electrophoresis.

3. Biochemistry and Molecular Biology: Functions as a buffering component in various reagent kits (e.g., PCR, DNA extraction); widely utilized in diagnostic reagents and biosynthesis.