| Name | 3,4-Dihydroxybenzylamine hydrobromide |
| Description | 3,4-Dihydroxybenzylamine hydrobromide (NSC-263475 hydrobromide) inhibits DNA polymerase activity in melanoma cells and displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity. |
| Cell Research | Cell Line: Human and Murine Melanoma Cell Lines; Non-Melanoma Cell Lines
Concentration: 1 μM-10 mM
Incubation Time: 48 hours [1] |
| Animal Research | Animal Model: C57BL/6 mice with B6D2F1 cells
Dosage: 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg
Administration: Intraperitoneal injection [2] |
| In vitro | 3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) inhibits the growth of melanoma cell lines with IC50 values of 30, 84, 90, and 68 μM for SK-MEL-30, SK-MEL-2, SK-MEL-3, and RPMI-7951 cells, respectively, and exhibits IC50 values of 10, 25, 67, and 184 μM for S91A, S91B, L1210, and SCC-25 cells. Cytotoxicity in SK-MEL-2 and SK-MEL-28 is enhanced by buthionine sulfoximine (BSO), decreasing the IC50 values of 3,4-DHBA by 127% in SK-MEL-28 and by 148% in SK-MEL-2 cells[1]. |
| In vivo | 3,4-Dihydroxybenzylamine hydrobromide (200, 400, 600 and 800 mg/kg; i.p.; 21 days) has different effects at different doses, the median life-span are 17, 24.5, 26, 29 and 25 days respectively. 3,4-Dihydroxybenzylamine hydrobromide (1000 mg/kg; i.p.; 7 days) has the least toxic effect in non-tumor-bearing B6D2F1 mice,and mice is tolerated at this dose[2]. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 80 mg/mL (363.54 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (15 mM), Sonication is recommended.
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| Keywords | tyrosinase | Tumor | RNASynthesis | RNA Synthesis | NSC-263475 Hydrobromide | NSC263475 Hydrobromide | NSC-263475 | NSC263475 | NSC 263475 Hydrobromide | NSC 263475 | melanoma | Inhibitor | inhibit | DNASynthesis | DNA/RNA Synthesis | DNA Synthesis | DNA polymerase | 3,4-Dihydroxybenzylamine Hydrobromide | 3,4Dihydroxybenzylamine hydrobromide | 3,4-Dihydroxybenzylamine | 3,4 Dihydroxybenzylamine hydrobromide |
| Inhibitors Related | 5-Fluorouracil | Adenine hemisulfate | Erythromycin thiocyanate | Guanidine hydrochloride | Hexane-1,6-diol | 1,4-Naphthoquinone | Adenine | Vidarabine | Carbazole | Thymidine | Docosanoic acid | Usnic Acid |
| Related Compound Libraries | DNA Damage & Repair Compound Library | Bioactive Compound Library | Inhibitor Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Cell Cycle Compound Library | Anti-Cancer Compound Library |
United States
