3,5-Dinitrosalicylic acid Cas 609-99-4
Product Description
Product Name: 3,5-Dinitrosalicylic acid
Synonyms: 3,5-Dinitro-2-hydroxybenzoic acid
CAS No. 609-99-4
M.F.: C7H4N2O7
M.W.: 228.12
EINECS: 210-204-3
Specifications
| Item | Specification |
| Appearance | Yellow Powder |
| Assay | ≥98.0% |
| Density | 1.7914 (rough estimate) |
| Melting Point | 168-172 °C (lit.) |
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3,5-Dinitrosalicylic acid is an organic aromatic compound used primarily as a reagent for the qualitative and quantitative detection of reducing sugars. It is structurally salicylic acid with two nitro groups (-NO₂) at the 3 and 5 positions of the benzene ring.
Primary Use: Reducing Sugar Assay
Its most important application is in the DNS Assay (or DNS Method), a common colorimetric test in biochemistry, food science, and biotechnology.
How it works: Under alkaline conditions, the nitro groups of DNS are strong oxidizing agents. When heated with a reducing sugar (like glucose, maltose, or fructose), the sugar is oxidized, and the DNS itself is reduced.
The result: This reduction converts the yellow-colored DNS into 3-amino-5-nitrosalicylic acid, which is a water-soluble compound with a deep orange-red/brownish-red color.
Quantification: The intensity of this red color, measurable with a spectrophotometer, is directly proportional to the concentration of reducing sugars in the sample.
Common applications of the DNS Assay include:
Measuring sugar concentration in biological fluids, food products, or beverages.
Determining enzyme activity (e.g., of amylase, cellulase, or invertase) by measuring the amount of sugar released from their substrates over time.
Monitoring progress in fermentation or sugar hydrolysis processes.
Other Uses and Properties
Analytical Chemistry: Serves as a reagent for detecting other reducing substances besides sugars.
Organic Synthesis: Can act as a precursor or intermediate in the synthesis of more complex organic compounds, including some dyes and pharmaceuticals.
pH Indicator: Its distinct color change also allows it to function as a crude pH indicator, transitioning from yellow (acidic) to red (alkaline).
Historical Use: It was once explored as a topical antiseptic (similar to other nitrated phenols) but is not commonly used for this purpose today.
Key Characteristics & Safety
Appearance: Typically a yellow crystalline powder.
Solubility: Soluble in water and alcohols.
Safety: It is an irritant to the skin, eyes, and respiratory system. Proper lab safety protocols (gloves, goggles) are required when handling it.
In summary, 3,5-dinitrosalicylic acid is a cornerstone laboratory reagent whose primary and most valuable function is the colorimetric measurement of reducing sugars via the classic DNS Assay.
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Different products have different MOQ, such as:
1).Peptide products, the MOQ is 1 gram level
2).Liquid products, the MOQ is usually 50-100ML
3).API, the MOQ is 1KG
4).Intermediate products and conventional product, the MOQ is 1KG; custom-tailored products, the MOQ depends on the production process.
5).Food additives and vitamin, the MOQ is usually one package: such as Vitamin C is 25KG / drum, Vitamin E oil or Vitamin A oil is 5KG/bag;Vitamin E powder or Vitamin A powder is 20KG / cartons.
6).Agrochemical , the MOQ is 1KG, except preparations.
6.How do we take the quality complaint ?
First of all, our quality control will reduce the quality issue to near zero. If there is a quality problem caused by us, we will send you free goods for replacement or compensate your loss.
Company Information
Company profile
Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical & its intermediates, Food/Feed additives, Fine chemicals in distributing the products of our own company and affiliated enterprises, are the members of Hubei E-commerce Chamber.
Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients’ requirement.
We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.
Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.
We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward powder, their satisfaction is our struggling goal.
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