| Name | 4-Aminohippuric Acid |
| Description | 4-Aminohippuric Acid is the glycine amide of 4-aminobenzoic acid. Its sodium salt is used as a diagnostic aid to measure effective renal plasma flow (ERPF) and excretory capacity. |
| In vitro | In normal individuals, the renal extraction ratio of 4-Aminohippuric acid is approximately 0.92. After extraction, the majority of 4-Aminohippuric acid is actively secreted by the proximal tubules and completely excreted by the kidneys. The clearance of 4-Aminohippuric acid is utilized to measure renal plasma flow and to assess the secretory mechanisms or transport limits of renal tubules. The kinetics of 4-Aminohippuric acid uptake by cortical tubular cells have been measured using the stopped-flow microperfusion technique in rat kidneys. In a rat model of renal failure, intravenous injection of 3.0 mg/kg Uranyl nitrate significantly reduced the CLPAH to 0.89 mL/min/kg, compared to 29.30 mL/min/kg in normal rats. Rats with chronic kidney failure exhibited altered pharmacokinetics, showing decreased renal excretion of 4-Aminohippuric acid. Compared to Sham rats, those with chronic kidney failure demonstrated a weakened load of excretion, filtration, and secretion of 4-Aminohippuric acid, with about a 40% reduction in the expression of OAT1 in the homogenate and membrane. Hence, measuring the renal clearance of 4-Aminohippuric acid typically reflects the kidney's capacity for organic anion transport. |
| In vivo | At a concentration of 10 mmol/L, 4-Aminohippuric acid was transported across membranes of HEK293 cells expressing MRP2 with a rate of 21.9 pmol/mg protein/min. The kinetic parameters for 4-Aminohippuric acid transport included a Km value of 880 mM and a Vmax value of 2.3 nmol/mg protein/min, indicating facilitated transport through organic anion transporters expressed in epithelial cells. Additionally, 4-Aminohippuric acid uptake by HEK293 cells, mediated by overexpressed and transfected NPT1, exhibited saturable kinetics in line with Michaelis-Menten dynamics, having a Km close to 2.66 mM and a Vmax of 940 pmol/mg protein/30s. This compound is also transported by transporters other than OAT, including NPT1 and MRP2. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 50 mg/mL (257.48 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (10.3 mM), Sonication is recommended.
Ethanol : 2 mg/mL (10.3 mM), Sonication is recommended.
H2O : 3 mg/mL (15.45 mM), Sonication is recommended.
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| Keywords | Inhibitor | inhibit | Endogenous Metabolite | AminoAcidsandDerivatives | Amino Acids and Derivatives | 4-Aminohippuric Acid | 4Aminohippuric Acid | 4 Aminohippuric Acid |
| Inhibitors Related | Sucrose | Aceglutamide | Nicotinamide riboside malate | DL-Lysine | D(+)-Raffinose pentahydrate | Guanidine hydrochloride | Malic acid | Formamide | Glycerol | Thymidine | Corn starch | Gluconate Calcium |
| Related Compound Libraries | Food Sourced Compound Library | Bioactive Compound Library | ReFRAME Related Library | Natural Product Library | Drug-Fragment Library | Natural Product Library for HTS | RO5 Drug-like Natural Product Library | Human Endogenous Metabolite Library | Human Endogenous Metabolite Compound Library Plus | Bioactive Compounds Library Max | Human Metabolite Library |
United States
