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ChemicalBook Product Catalog Biochemical Engineering Saccharides Monosaccharide 4-MUNANA

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4-MUNANA NEW

$31.00 1mg

$80.00 5mg

$126.00 10mg

Min. Order
Purity98.02%
Cas No76204-02-9
Supply Ability10g
Update time2026-04-14

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

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United States

United States

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Chemical Properties

Product Name	4-MUNANA
CAS No	76204-02-9
EC-No
Min. Order
Purity	98.02%
Supply Ability	10g
Release date	2026/04/14

Product Introduction

Bioactivity

Name	4-MUNANA
Description	4-MUNANA is a fluorescent substrate employed in neuraminidase activity assays and serves as a neuraminidase substrate analogue.
In vitro	Instructions: I. Solution preparation 1. Stock solution: Dissolve 4-MUNANA in a suitable buffer to prepare a stock solution with a concentration of 1–10 mM. The specific concentration can be adjusted according to the experimental requirements. 2. Working solution: Dilute the 4-MUNANA stock solution to a working concentration (usually 100–500 µM). The specific concentration can be adjusted according to the experimental requirements. Notes: Powder: Store at -20°C or lower in a dark, dry environment. Stock solution: Store at -20°C in a dark place after aliquoting. Avoid repeated freezing and thawing. Store at 4°C for a short period of time. II. Operation steps Neuraminidase activity detection 1. Detection buffer: Prepare a buffer suitable for neuraminidase activity, such as 50 mM sodium acetate (pH 5.5). 2. Enzyme reaction: Mix a sample containing neuraminidase (such as a virus sample or cell lysate) with the 4-MUNANA working solution. 3. Incubate at 37°C for 30–60 Minutes (time can be optimized according to the experiment). 4. After the reaction is completed, add an equal volume of stop solution (such as 0.1 M glycine buffer, pH 10.4) to enhance the fluorescence signal. 5. Fluorescence detection Detection method: Use a fluorescence spectrophotometer or microplate reader to detect the fluorescence signal generated by the reaction. Excitation wavelength: ~365 nm Emission wavelength: ~450 nm Notes Standard curve: Use known concentrations of 4-methylumbelliferone to prepare a standard curve for quantitative analysis of neuraminidase activity. Control experiment: Set up negative control (no enzyme) and positive control (containing known inhibitors) to verify the specificity of the detection. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMF : 15 mg/mL (30.65 mM), Sonication is recommended. H2O : 80 mg/mL (163.46 mM), Sonication is recommended. Ethanol : 0.25 mg/mL (0.51 mM), Sonication is recommended. PBS (pH 7.2) : 10 mg/mL (20.43 mM), Sonication is recommended. DMSO : 255 mg/mL (521.04 mM), Sonication is recommended.
Keywords	4MethylumbelliferylNacetylαDNeuraminic Acid (sodium salt) \| 4-Methylumbelliferyl-N-acetyl-α-D-Neuraminic Acid \| 4-Methylumbelliferyl-N-acetyl-a-D-Neuraminic Acid \| 4 Methylumbelliferyl N acetyl α D Neuraminic Acid (sodium salt)

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