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ChemicalBook Product Catalog Chemical Reagents Organic reagents Tricyclic compound 5-FAM

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5-FAM NEW

$33.00 50mg

$47.00 100mg

Min. Order
Purity98.28%
Cas No76823-03-5
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP4Y

United States

United States

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Chemical Properties

Product Name	5-FAM
CAS No	76823-03-5
EC-No
Min. Order
Purity	98.28%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	5-FAM
Description	5-FAM (5-Carboxyfluorescein) (5-Carboxyfluorescein) contains a carboxylic acid, which can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its excellent fluorescence quantum yield, relatively high absorptivity, and good water solubility, it also has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.
Cell Research	Instructions 1. Preparation and storage a. Solubility: 5-FAM is usually provided in powder form and is easily soluble in DMSO, DMF, or aqueous solution with pH ≥ 8. b. Recommended stock solution concentration: 10–100 mM (adjusted according to experimental needs). Before use, it is recommended to prepare with anhydrous solvent and dispense in small volumes to avoid repeated freezing and thawing. 2. Biomolecule labeling (such as proteins, nucleic acids) a. Activation reaction: 5-FAM is usually combined with an activation reagent (such as EDC/NHS) to generate active esters to facilitate reaction with the amine group of the target molecule. b. Amine-free neutral buffer (such as MES or PBS, pH 6–8) is recommended for the reaction buffer. c. Binding step: Mix the active ester with the target molecule and react at room temperature for 1–2 hours. Optimize the pH and reaction time if necessary. d. Purification: Use dialysis or gel filtration to remove unreacted 5-FAM. 3. Fluorescence imaging a. Sample preparation: dilute 5-FAM to working concentration (usually 1–10 µM). b. Directly apply or incubate cells or tissue for 10–30 minutes, followed by washing with PBS to remove unbound dye. c. Microscope observation: Excitation wavelength: ~495 nm. Emission wavelength: ~520 nm (green fluorescence). The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 200 mg/mL (531.46 mM), Sonication is recommended.
Keywords	Inhibitor \| inhibit \| 5-FAM \| 5FAM \| 5 FAM
Related Compound Libraries	Human Metabolite Library

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