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5-IAF NEW

$31.00 1mg

$64.00 5mg

Min. Order
Purity
Cas No63368-54-7
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP4Y

United States

United States

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Chemical Properties

Product Name	5-IAF
CAS No	63368-54-7
EC-No
Min. Order
Purity
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	5-IAF
Description	5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains). 5-IAF can label the catalytic (alpha) subunit of dog kidney Na,K-ATPase.
In vitro	Instructions 1. Preparation and storage a. Solubility: 5-IAF is usually provided in powder form and is easily soluble in DMSO, DMF or aqueous solution at pH ≥ 8. Recommended stock solution concentration: 10–50 mM (adjust according to experimental needs). Before use, dissolve it in anhydrous solvent and aliquot into small volumes to avoid repeated freezing and thawing. b. Storage conditions: Powder: Store in a dry environment, away from light at -20°C or lower. Solution: Short-term storage at 4°C, long-term storage at -20°C away from light is recommended. 2. Thiol labeling a. Protein reduction treatment (if necessary): To ensure that the thiol groups are in a reduced state, protein samples can be pretreated with DTT or TCEP. Use a thiol-free buffer (such as PBS or HEPES), pH 7–8 is the optimal range. b. Labeling reaction: Dilute the stock solution of 5-IAF to an appropriate working concentration (usually 0.1–1 mM). Add to the protein solution (usually 1–10 mg/mL) and mix gently. c. Incubate at room temperature for 30 minutes to 2 hours, the specific time needs to be optimized according to the experiment. d. Termination reaction: Terminate the labeling reaction by adding excess reducing agent (such as β-mercaptoethanol) or dilution. e. Purification: Remove unbound 5-IAF and reaction byproducts using dialysis, gel filtration, or centrifugal ultrafiltration. 3. Fluorescence detection Excitation wavelength: ~495 nm. Emission wavelength: ~520 nm (green fluorescence). The fluorescent signal of the labeled protein can be detected in a fluorescence microscope or fluorescence spectrometer. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMF : 90 mg/mL (174.67 mM), Sonication and heating to 60℃ are recommended. DMSO : 45 mg/mL (87.34 mM), Sonication is recommended.
Keywords	5-IAF

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