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ChemicalBook Product Catalog Biochemical Engineering Biochemical Reagents Amino Acids and Proteins 6-TAMRA

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6-TAMRA NEW

$41.00 10mg

$74.00 25mg

$116.00 50mg

Min. Order
Purity98.23%
Cas No91809-67-5
Supply Ability10g
Update time2026-04-14

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

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United States

United States

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Chemical Properties

Product Name	6-TAMRA
CAS No	91809-67-5
EC-No
Min. Order
Purity	98.23%
Supply Ability	10g
Release date	2026/04/14

Product Introduction

Bioactivity

Name	6-TAMRA
Description	6-TAMRA (6-Carboxytetramethylrhodamine) serves as a popular fluorophore for the creation of bioconjugates, particularly in the synthesis of fluorescent antibodies and avidin derivatives employed in immunochemistry.
In vitro	6-TAMRA labeled nucleotide experiment Operation steps 1. Sample treatment: Incubate in NHOH/methylamine (1:1) solution at 65°C for 10 minutes to separate oligonucleotides from CPG. 2. Take the supernatant and wash CPG with 1 mL EtOH/MeCN/H2O (3:1:1). 3. Mix the supernatants and dry them. 4. Treat with fresh anhydrous triethylammonium fluoride/n-methylpyridone (250 μL, n-methylpyridone 1.5 mL, triethylamine 750 μL, tea-3hf 1.0 mL) at 65°C for 1.5 h to remove t-butyl-dimethylsilyl protecting groups from RNA residues, and add 25 μL 3 M NaOAc and 1 mL n-BuOH to precipitate oligonucleotides. 5. The sample was cooled at -70°C for 1 h and then centrifuged at 10,000 g for 30 min. The supernatant was decanted, washed with aqueous EtOH (70% v/v), and then dried. 6. 6-TAMRA (0.1 mL, 10 mg/mL in dimethyl sulfoxide) was added to the 3'-amino-modified oligonucleotide suspended in 1.0 mL of sodium bicarbonate buffer (pH 8.5) and incubated at 37°C for 12 h. 7. The labeled oligonucleotide was resuspended in water and passed through a G25 Nap-10 disposable desalting column to remove free dye. 8. The oligonucleotide was purified by HPLC with a linear gradient of acetonitrile in 0.1 M triethylammonium acetate (TEAA) buffer, pH 7.2. 9. The entire sample was loaded on a Hamilton PRP-1 column and eluted with a linear gradient of acetonitrile for 40 min. Samples were monitored at 260 and 297 nm, and peaks corresponding to the dual-labeled oligonucleotide species were collected. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 2.86 mg/mL (6.64 mM), Sonication is recommended.
Keywords	Inhibitor \| inhibit \| 6-TAMRA \| 6TAMRA \| 6 TAMRA
Inhibitors Related	Stavudine \| Aceglutamide \| Hemin \| Tamoxifen \| Cysteamine hydrochloride \| Guanidine hydrochloride \| Hydroxychloroquine \| Enzalutamide \| Paeonol \| Naringin \| Alginic acid \| Sildenafil citrate

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