| Name | Adropin (34-76) (human, mouse, rat) |
| Description | Adropin (34-76) (human, mouse, rat) is a peptide that attenuates liver fibrosis and insulin resistance independent of obesity or food intake. |
| In vitro | Method: HUVECs cells were treated with 10 ng/mL Adropin for 18 hours, and then Adropin was added to the culture medium; in addition, HUVECs cell line proteins were isolated, and Adropin (10 ng/mL) was added to the culture medium to stimulate for 0 to 60 minutes to lyse the treated HUVECs. The samples were used to measure phosphorylated ENOs, phosphorylated AKT, and phosphorylated ERK1/2.
RESULTS Adropin-treated HUVECs cells showed greater proliferation, migration, and capillary-like tube formation; Adropin upregulated endothelial NO synthase expression through the VEGFR2 2-PI3K-Akt and VEGFR2-extracellular signal-regulated kinase pathways to reduce inflammation. [3]
Method: Treat 3T3-L1 cells or rat primary preadipocytes with Adropin (1 - 100 nmol/l) for 24 or 48 hours. After that, add BrdU solution (10µmol/l), incubate the cells for 3 hours, and use Cell Proliferation BrdU Elisa Kit (Roche Diagnostics, Penzberg, Germany) evaluates the binding of BrdU to DNA.
RESULTS Adropin (100 nmol/l) stimulated the proliferation of 3T3-L1 preadipocytes after 24 and 48 hours. Adpin at a concentration of 100 nmol/l increased the proliferation of rat preadipocytes after 48 hours; in addition, Adropin stimulated the proliferation of rat preadipocytes through ERK1 /2 and AKT-dependent mechanisms promote 3T3-L1 cell proliferation. [4] |
| In vivo | METHODS: DIO C57BL/6 mice maintained on a 60% kcal fat diet received five intraperitoneal (ip) injections of adropin (450 nmol/kg). After treatment, assess glucose tolerance and systemic insulin sensitivity.
RESULTS Adropin treatment improved glucose tolerance, enhanced insulin action, and enhanced metabolic flexibility in glucose utilization in mice. [1]
METHODS: C57Bl/6 mice were injected with secretable form of Adropin (450 nmol/kg, ip) three times within 24 hours, then fasted, and the hearts were isolated and perfused.
RESULTS Adropin administration improved cardiac function, cardiac efficiency, and coronary blood flow compared with untreated mice. [2] |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Inhibitors Related | Undecane | Aceglutamide | Musk ketone | Stearamide | Bisphenol A | Rhamnose monohydrate | Hyaluronic acid | Ethyl linoleate | Methyl eugenol | Chloramphenicol | 2,3-Butanediol | Isovaleric acid |
United States
