| Name | AkaLumine hydrochloride |
| Description | AkaLumine hydrochloride is a fluorescein analog with a Km value of 2.06 μM for recombinant fluorescent protein. |
| Animal Research | Instructions
I. Solution preparation
1. Stock solution preparation Dissolve AkaLumine hydrochloride in deionized water or dimethyl sulfoxide (DMSO).
2. Working solution preparation: Prepare a stock solution of no more than 50 mg/mL, with a common concentration of 40 mg/mL. For example, 40 mg AkaLumine hydrochloride can be dissolved in 1 mL deionized water or DMSO.
Notes: Storage: It is recommended to prepare and use immediately; if storage is required, it should be stored at low temperature to ensure stability.
II. In vivo imaging:
1. Administration: Inject the prepared AkaLumine hydrochloride solution into experimental animals by intraperitoneal injection.
2. Dosage: The specific dose is adjusted according to the experimental situation, generally ranging from 50–200 mg/kg.
3. Imaging: After injection, use an imaging system that can detect NIR luminescence for bioluminescence imaging.
The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| In vitro | In both LLC/luc and MDA-MB-231/Luc cells exposed to AkaLumine hydrochloride, the signal peaks at lower concentrations (starting from 2.5 μM). Conversely, the bioluminescence produced by D-luciferin and CycLuc1 continuously increases with concentration, without reaching saturation, even at 250 μM.[1] |
| In vivo | AkaLumine hydrochloride-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, exhibit a 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with the experimental control group. In the inverse order at an 8 h interval. AkaLumine hydrochloride displays about a fourfold increase in signals from lung metastasis compared with the experimental control group.[1] |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween-80+45% Saline : 5 mg/mL (14.76 mM), Sonication is recommended.
DMSO : 120 mg/mL (354.14 mM), Sonication is recommended.
H2O : 25 mg/mL (73.78 mM), Sonication is recommended.
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| Keywords | recombinant Fluc protein | Dye reagent | AkaLumine Hydrochloride | 1176235-08-7 free base | 1176235-08-7 |
United States
