Basic information Safety Related Supplier

ChemicalBook Product Catalog Amine-PEG3-Biotin

1/1

Amine-PEG3-Biotin NEW

$29.00 25mg

$49.00 50mg

Min. Order
Purity99.76%
Cas No359860-27-8
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

show more

Enterprise Verified

Business Bank account Basic Contact Infomation Business Address Trade Company

Chemical Properties

Product Name	Amine-PEG3-Biotin
CAS No	359860-27-8
EC-No
Min. Order
Purity	99.76%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	Amine-PEG3-Biotin
Description	Amine-PEG3-Biotin can be used as a signal amplification label.
Cell Research	1. Binding to biological molecules 1. Labeling reaction: Amine-PEG3-Biotin reacts with the target biological molecule, usually by amidation reaction to connect the dye molecule to the target molecule. Depending on the desired labeling effect, the reaction conditions, such as pH, reaction time and temperature, may need to be optimized. 2. Purification of labeled molecules: After the reaction is completed, unreacted biotin is usually removed by dialysis or chromatography. 3. Detection: Detection is performed using streptavidin-enzyme or streptavidin-fluorescent markers to amplify the signal for analysis. 2. Western Blot and immunoassay 1. Labeling antibodies: Enhance the detection sensitivity by labeling Amine-PEG3-Biotin with primary or secondary antibodies. 2. Detection: The labeled antibody is used for protein transfer and detection is performed using streptavidin-enzyme or streptavidin-fluorescent markers. 3. Signal amplification: The strong binding of biotin to streptavidin enables the signal to be amplified, improving the sensitivity of immunoassay. III. ELISA (Enzyme-Linked Immunosorbent Assay) 1. Coating: Coat the biotin-labeled target molecule onto the ELISA plate (such as a labeled antibody or antigen). 2. Detection: Detection is performed by adding streptavidin-enzyme conjugates and generating color or signal through substrate reaction. IV. Cell experiment 1. Labeling cells: Add Amine-PEG3-Biotin to the cell culture medium and incubate for a certain period of time (usually 30 minutes to 1 hour). 2. Washing: Wash away unbound dye with PBS. 3. Fluorescence microscopy: Use a fluorescence microscope or flow cytometer for detection to observe the staining pattern and dynamic process of the cells. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Store at -20°C Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	H2O : 95 mg/mL (226.97 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween-80+45% Saline : 1 mg/mL (2.39 mM), Sonication is recommended. DMSO : 245 mg/mL (585.35 mM), Sonication is recommended.
Keywords	Inhibitor \| inhibit \| Amine-PEG3-Biotin \| Amine-PEG-3-Biotin \| AminePEG3Biotin \| Amine PEG3 Biotin

show more

Company Profile Introduction

You may like

Recommended supplier

Send Inquriy