| Name | Apocynin |
| Description | Apocynin (NSC 2146) is a specific NADPH-oxidase inhibitor (IC50: 10 μM). |
| In vitro | Apocynin demonstrates anti-inflammatory activity by reducing neutrophil infiltration, the extent of spinal cord injury, the formation of PAR and nitrotyrosine, and the expression of ICAM-1 and P-selectin. It inhibits the degradation of IκB-α, activation of NF-κB, and apoptosis (as indicated by TUNEL staining, FAS ligand, Bax, and Bcl-2 expression), and the production of pro-inflammatory cytokines (TNF-α and IL-1β) and the activation of MAPK pathways (P-38 and phosphorylated-JNK). The LD50 in mice is 9 g/kg (p.o.). Apocynin is effective in various inflammatory cells and animal models. It attenuates the production of TNF-α and IL-1β, and the expression of iNOS in the lungs of mice treated with carrageenan. Moreover, apocynin offers beneficial effects in mouse models of spinal cord injury. |
| In vivo | Upon metabolic transformation, Apocynin can inhibit the assembly of NADPH oxidase, possibly due to the production of reactive oxygen species (ROS). It achieves this by inhibiting the serine phosphorylation of p47phox and disrupting its interaction with gp91phox, thereby inactivating NADPH oxidase activity. Furthermore, Apocynin reduces the generation of superoxide (O(2)(-)) in activated macrophages and neutrophils. |
| Storage | 02 | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 250 mg/mL (1504.48 mM), Sonication is recommended.
Ethanol : 16.6 mg/mL (99.9 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (12.04 mM), Sonication is recommended.
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| Keywords | NSC-2146 | NSC2146 | NADPH oxidase | Inhibitor | inhibit | Autophagy | Apoptosis | Apocynin |
| Inhibitors Related | Stavudine | Aceglutamide | Hemin | Urea | Tamoxifen | Guanidine hydrochloride | Hydroxychloroquine | Metronidazole | Formamide | Paeonol | Naringin | Alginic acid |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Bioactive Compound Library | Traditional Chinese Medicine Monomer Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Natural Product Library | Tobacco Monomer Library | Inhibitor Library | Natural Product Library for HTS | Immunology/Inflammation Compound Library | Heat-Clearing and Detoxifying Traditional Chinese Medicine Compound Library | Ancient Chinese Classical Formulas Compound Library | Food as Medicine Compound Library |
United States
