| Name | Asperosaponin VI |
| Description | Asperosaponin VI (Akebia saponin D) , the most abundant constituent of the rhizome of Dipsacus asper, has been used for the treatment of lower back pain, traumatic hematoma and bone fractures. |
| Kinase Assay | Cells are grown on 12-mm diameter glass coverslips in DMEM 100% serum and various concentrations of KN-93 or KN-92. After 0, 1, 2, and 3 days of culture in the presence of drug, coverslips are removed from culture, rinsed once in PBS, and then submerged in 100% methanol at -20°C for 3 min. Fixed cells are stored in PBS until staining using the TUNEL assay. Cells are overlaid on 20 μL PBS/1 mg/mL BSA for 30 min, rinsed in PBS, and then overlaid on 20 μL containing 100 mM sodium cacodylate (pH 6.8), 1 mM CoCl2, 0.1 mM DTT, 0.1 mg/mL BSA, 20 μM fluorescein-12-dUTP, and 0.1 unit/μL terminal transferase at 37°C for 60 min. Coverslips are rinsed in PBS twice, mounted on slides, and photographed using an OLYMPUS BX5O epifluorescent microscope using a UPLAN APO 40X oil immersion objective. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.30 mg/mL (3.55 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble)
DMSO : 99.41 mg/mL (107.00 mM), Sonication is recommended.
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| Keywords | Inhibitor | inhibit | Caspase | Asperosaponin VI | Apoptosis |
| Inhibitors Related | Stavudine | Aceglutamide | Urea | Tamoxifen | Cysteamine hydrochloride | Daidzein | Metronidazole | Ethyl linoleate | Formamide | Daraxonrasib | Alginic acid | Sildenafil citrate |
| Related Compound Libraries | Terpene Natural Product Library | Traditional Chinese Medicine Monomer Library | Membrane Protein-targeted Compound Library | Selected Plant-Sourced Compound Library | Miao medicine Compound Library | Saccharide and Glycoside Natural Product Library | Natural Product Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max | Heat-Clearing and Detoxifying Traditional Chinese Medicine Compound Library | Ancient Chinese Classical Formulas Compound Library | Food as Medicine Compound Library |
United States
