Avidin(chiken egg white)

English Name: avidin (chiken egg white)

Other names: avidin; Avidin; Affinity element

CAS No.: 1405-69-2

Molecular weight: glycoprotein mol wt 66 kDa; subunit mol wt 16 kDa

Grade: BR

Protein：≥95.0%

Activity: 10-15 units / mg protein (E1% / 280)

Definition of vitality: one unit will bind 1.0 μ g of d-biotin

Solubility (5% water): Pass

Physicochemical properties: white powder, it is a glycoprotein. Since each molecule is composed of 4 subunits, it can closely bind with 4 biotin molecules. pI 10～10.5； It is stable in pH 9-13 buffer, heat-resistant and resistant to the action of many proteolytic enzymes. Generally, the active unit of the affinity element is expressed by the amount of affinity element binding biotin, that is, the amount of affinity element required to bind 1Pg biotin is one affinity element active unit. Generally, 1Pg affinin contains about 13-15 active units

Use: biochemical research, can combine with biotin (vitamin H) to make it lose its function.

Storage: 2 ~ 8 ℃