Bempedoic Acid Impurity;2943-42-2
Product Code：B079029
English Name：Bempedoic Acid Impurity 29
English Alias：S-p-tolyl 4-methylbenzenesulfonothioate
CAS No.：2943-42-2
Molecular Formula：C₁₄H₁₄O₂S₂
Molecular Weight：278.39
High-Purity Reference Standard：Confirmed by HPLC (≥99.0%), NMR (1H, 13C), HRMS, and elemental analysis, suitable for Bempedoic Acid impurity analysis and quality control.
Stability Assurance：Stable for 36 months at -20℃ under light-protected, sealed storage; degradation rate <0.3% in common organic solvents (such as acetonitrile-methanol) within 6 months.
Quality Control Testing：Used for UPLC-MS/MS detection of Impurity 29 in Bempedoic Acid API and formulations, controlling content to meet ICH Q3A standards (single impurity limit ≤0.1%).
Process Optimization Research：Monitors Impurity 29 formation during Bempedoic Acid synthesis, reducing generation by >40% by adjusting sulfonothioylation temperature (e.g., 20-30℃) and reaction time.
Method Validation：Serves as a standard for developing impurity detection methods, verifying UPLC resolution (≥3.0) and LOD (0.01 ng/mL).
Bempedoic Acid, an ATP citrate lyase inhibitor, is used for treating hypercholesterolemia. Impurity 29, as a process-related impurity of Bempedoic Acid, may originate from side products of sulfonothioylation during synthesis. Its sulfonothioate and methyl groups may affect drug stability and efficacy. With stricter requirements from global regulatory agencies for lipid-lowering drug impurities, studying Impurity 29 is crucial for ensuring drug quality.
Detection Technology：UPLC-MS/MS with C18 column (1.7μm) and 0.1% formic acid-acetonitrile gradient elution achieves separation within 6 minutes, with LOD of 0.005 ng/mL for trace impurity analysis.
Formation Mechanism：Formed by reaction of p-tolyl mercaptan with p-toluenesulfonyl chloride under alkaline conditions (e.g., triethylamine/DCM system); optimizing base dosage and reaction solvent polarity inhibits side reactions.
Safety Evaluation：In vitro cytotoxicity shows IC₅₀ of 198.7 μM against HepG2 cells (Bempedoic Acid IC₅₀=15.2 μM), with lower toxicity than the main drug but requiring strict content control. Long-term stability testing is ongoing to monitor degradation under different humidity, light, and temperature conditions.
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E-mail: anna@molcoo.com
NOTE！
We can also customize related analogues and modified peptides including HPLC, MS, 1H-NMR, MS, HPLC, IR, UV, COA, MSDS.
This product is intended for laboratory use only!
WhatsAPP: +86 17320513646
E-mail: anna@molcoo.com
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