| Name | BH3I-1 |
| Description | BH3I-1 (BHI1) is a Bcl-2 antagonist. |
| Cell Research | Cells (5×104 cells per well) are seeded into white 96-well plates (Costar) and treated with various concentrations of the compounds for 48 h. For zVAD-FMK protection experiments, cells are preincubated with 100 μM zVAD-FMK for 1 h before the addition of chemicals. Cell viability is determined with an MTS assay. For PI staining experiments, cells are grown in 24-well plates and then incubated with 2 μg ml/L PI. Cell death is determined by FACS analysis.(Only for Reference) |
| In vitro | BH3I-1 inhibits Bcl-xL heterodimerization in vitro. It also induces cytochrome c release[1]. BH3I-1, while inhibiting its reported target Bcl-2/Bim and Bcl-xL/Bim, shows significant inhibition of both the p53/hDM2 and p300/Hif-1a interactions[2]. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 60 mg/mL (149.88 mM), Sonication is recommended.
Ethanol : 11 mg/mL (27.48 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (5 mM), Sonication is recommended.
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| Keywords | Ubiquitin ligase | Ubiquitin conjugating enzyme | Ubiquitin activating enzyme | p53/MDM2 | MDM-2/p53 | Inhibitor | inhibit | E3Enzyme | E3 ligating enzyme | E3 Enzyme | E2Enzyme | E2 Enzyme | E2 conjugating enzyme | E1Enzyme | E1/E2/E3 Enzyme | E1 Enzyme | E1 activating enzyme | BHI-1 | BHI 1 | BH3I-1 | BH-3I-1 | BH3I1 | BH3I 1 | BH 3I1 | Bcl-xL-BH3 | Bcl-2 Family |
| Inhibitors Related | Cyanoacetamide | 10-Hydroxydecanoic Acid | Flubendazole | Pendimethalin | Amantadine | Estradiol benzoate | D-(-)-3-Phosphoglyceric acid disodium | N-Hydroxyphthalimide | Venetoclax | Indole-3-carbinol | Methyl methanesulfonate | (E/Z)-10-Hydroxy-2-decenoic acid |
| Related Compound Libraries | Target-Focused Phenotypic Screening Library | Anti-Neurodegenerative Disease Compound Library | Bioactive Compound Library | HIF-1 Signaling Pathway Compound Library | Anti-Parkinson's Disease Compound Library | Anti-Cancer Metabolism Compound Library | Glutamine Metabolism Compound Library | Hematonosis Compound Library | Inhibitor Library | Mitochondria-Targeted Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max |
United States
