| Name | Boc-Leu-Gly-Arg-AMC |
| Description | Boc-Leu-Gly-Arg-AMC is a specific fluorogenic substrate designed for the enzymatic detection and quantification of complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme activity. Boc-Leu-Gly-Arg-AMC is also susceptible to hydrolysis by macropain, a high molecular weight thiol proteinase isolated from human erythrocytes, making it a versatile tool for enzymatic assays. |
| In vitro | To demonstrate the presence of proteolytic enzymes capable of producing sodifrin in the abdominal gland, polybutoxycarbo-nyl (Boc)-Leu-Gly-Arg-4-methylcoumaryl-7-amide (MCA) and Boc-Leu-Leu-Lys-MCA were used as synthetic substrates. Crude extracts of the abdominal gland hydrolyzed both substrates to release 7-amino-4-methylcoumarin, indicating the presence of enzymes in the gland that generate sodifrin from its precursor molecule. The activity of the extracts to cleave Boc-Leu-Gly-Arg-AMC was optimal at pH 9.0 and 45°C, while the activity to cleave Boc-Leu-Leu-Lys-MCA was optimal at pH 9.0 and 40°C. [1] |
| Storage | keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (5.48 mM), Sonication is recommended.
DMSO : 80 mg/mL (132.96 mM), Sonication is recommended.
H2O : < 0.1 mg/mL (insoluble), Sonication is recommended.
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| Keywords | Boc-Leu-Gly-Arg-AMC | BocLeuGlyArgAMC | Boc Leu Gly Arg AMC |
| Inhibitors Related | Boc-Lys(Boc)-OH | Aceglutamide | H-DL-Abu-OH | N-Methylsarcosine | DL-Proline | Sodium lauroyl glutamate | L-Ornithine L-aspartate | Fmoc-Aib-OH | Fmoc-L-aspartic acid | H-Glu(OEt)-OEt.HCl | Fmoc-His(Trt)-OH | Fmoc-Lys(Alloc)-OH |
| Related Compound Libraries | Bioactive Compound Library | Peptide Compound Library | Bioactive Compounds Library Max |
United States
