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ChemicalBook Product Catalog C12FDG

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C12FDG NEW

$ 163.00 /1mg

Min. Order
Purity98.99%
Cas No138777-25-0
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

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Chemical Properties

Product Name	C12FDG
CAS No	138777-25-0
EC-No
Min. Order
Purity	98.99%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	C12FDG
Description	C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry.
Cell Research	Fluorescence aging-related β-galactosidase (SA-β-Gal) experiment I. Solution preparation: 1. Mother solution preparation: Prepare a certain concentration of C12FDG mother solution, store it at -20℃ or -80℃ in the dark after aliquoting. 2. Working solution preparation: Select the appropriate working solution concentration according to the experimental requirements, and try to prepare it before use. II. Operation steps: 1. Culture cells with a density of 105 cells/mL 2. Wash the cells once with 200 μL PBS, and then fix them with 100 μL fixative (2% formaldehyde/0.2% glutaraldehyde distilled water) at room temperature for 5 minutes. 3. Wash the cells twice with 200 μL PBS, stain with 100 μL 33 μM C12FDG (PBS, pH = 6.0) for 10 min, and then stain with 200 μL Hoechst solution (1 μg/mL Hoechst 33342) in PBS (pH 6.0) for 10 min. d. Image the cells using a 20x objective and excitation filters of 360 nm (Hoechst 33342) and 480 nm (C12FDG), and monitor the cells using emission filters of 460 nm and 535 nm. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
In vitro	Fluorescence aging-related β-galactosidase (SA-β-Gal) assay [1]: 1.Cultivate cells at a density of 10^5 cells/mL. 2.Wash cells once with 200 μL PBS, then fix with 100 μL fixation solution (2% formaldehyde/0.2% glutaraldehyde in1. distilled water) at room temperature for 5 minutes. 3.Wash cells twice with 200 μL PBS, stain with 100 μL 33 μM C12FDG (PBS, pH 6.0) for 10 minutes, then stain with 200 μL Hoechst solution (1 μg/mL Hoechst 33342) in PBS (pH 6.0) for 10 minutes. 4.Image cells using a 20x objective and excitation filters of 360 nm (Hoechst 33342) and 480 nm (C12FDG), monitor cells using emission filters of 460 nm and 535 nm. This protocol is provided for reference and should be modified according to the specific requirements of your experiment. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 150 mg/mL (175.66 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween-80+45% Saline : 1.5 mg/mL (1.76 mM), Sonication is recommended.
Keywords	C12FDG

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