Human Cadherin-9 / CDH9 Protein, His Tag
Synonym
Cadherin-9, CDH9
Source
Human Cadherin-9, His Tag (CA9-H52H6) is expressed from human 293 cells (HEK293). It contains AA Gly 54 - Ala 615 (Accession # Q9ULB4-1).
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 64.5 kDa. The protein migrates as 70-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Background
Cadherin-9 (CDH9) is a member of the larger Cadherin superfamily of cell surface glycoproteins originally identified as proteins mediating cell-cell adhesion. Cadherins are a large family of cell –cell adhesion molecules involved in inter-cellular adhesion in a wide variety of cell types. In the nervous system, cadherins are known to be crucial to all stages of development, including the early separation of the neural tube from the ectoderm, the segregation of neurones and axons, and the formation of synapses.
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