| Name | D-Lin-MC3-DMA |
| Description | D-Lin-MC3-DMA, an ionizable cationic lipid, serves as a potent siRNA delivery vehicle. |
| Cell Research | White leghorn chicken embryos at stage 24 (30) were removed and dissected in Hanks buffered saline solution (HBSS).?Forelimbs were pooled and treated for 1 h in dispase to remove epithelial ectoderm.?Limb mesenchyme was dissociated, and resuspended in DMEM with 5% FBS and 1% penicillin/streptomycin.?Cells were plated onto 96-well plates at a density of 2x10^5 or 2x10^7 cells/ml and treated with 0-40 μg/ml LNP-pDNA.?Cultures were incubated overnight at 37℃ and resuspended in PBS buffer for expression analysis using a BD LSRII flow cytometer.?Cell survival was assessed in low-density cultures after 24 h of treatment, by counting the adherent cells remaining after PBS washes.?Viability was determined based on average counts of all adherent cells within a single field of view (100x) normalized to cell counts of untreated cultures. |
| In vitro | Replacement of DSPC by unsaturated PCs and DLin-MC3-DMA by the related lipid DLin-KC2-DMA resulted in highly potent transfection reagents for HeLa cells in vitro. |
| Storage | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility Information | Ethanol : 16.67 mg/mL (25.96 mM), Sonication and heating are recommended.
DMSO : 43.33 mg/mL (67.48 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (3.11 mM), Sonication is recommended.
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| Keywords | Inhibitor | inhibit | D-Lin-MC3-DMA | D-Lin-MC-3-DMA | DLinMC3DMA | D Lin MC3 DMA |
| Inhibitors Related | Stearamide | DOPE | DOTAP chloride | Dimethyldioctadecylammonium (bromide) | DMG-PEG 2000 | 1,2-Distearoyl-sn-glycero-3-phosphorylcholine | DODAP | Benzyl nicotinate | DSPS | 1,2-Dierucoyl-sn-glycero-3-PC | Cholesterol | Phosphatidylcholines,soya |
| Related Compound Libraries | Bioactive Compound Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Bioactive Lipid Compound Library | Anti-COVID-19 Compound Library |
United States
