| Name | Deoxycholic acid |
| Description | Deoxycholic acid (Cholanoic Acid) is a bile acid formed by bacterial action from cholate. It is usually conjugated with glycine or taurine. Deoxycholic acid acts as a detergent to solubilize fats for intestinal absorption, is reabsorbed itself, and is used as a choleretic and detergent. |
| Cell Research | Primary rat hepatocytes were isolated from male rats (100 to 150 g) by collagenase perfusion. After isolation, hepatocytes were resuspended in complete Williams E medium and plated on BD Primaria™ culture dishes at 5 × 104 cells/cm2. Cells are kept at 37 °C in a humidified atmosphere of 5% CO2 for 4-6 h to allow attachment. Plates are then washed with phosphate buffered saline (PBS) 1× in order to remove dead cells and incubated in Williams E medium supplemented with 25 to 200 μM DCA or no addition (control) for 24 h. Primary rat hepatocytes are processed for total RNA and protein isolation, cell viability, cytotoxicity and caspase activity assays and Hoechst staining. (Only for Reference) |
| In vitro | Deoxycholic acid inhibits miR-21 expression in primary rat hepatocytes in a dose-dependent manner, and increases miR-21 pro-apoptotic target programmed cell death 4 (PDCD4) and apoptosis. Deoxycholic acid decreases NF-κB activity, shown to represent an upstream mechanism leading to modulation of the miR-21/PDCD4 pathway[1]. |
| In vivo | miR-21 expression is down-regulated upon short exposures to Deoxycholic acid, as is the downstream pathway, with concomitant PIDD processing and activation of caspase-2, which contributes to DCA-induced liver damage[1]. |
| Storage | 02 | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 145 mg/mL (369.36 mM), Sonication is recommended.
Ethanol : 56 mg/mL (142.65 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble or slightly soluble)
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| Keywords | TGR5 | Inhibitor | inhibit | G-protein coupled receptor 19 | GPCR19 | GPBAR1 | G protein-coupled Bile Acid Receptor 1 | G protein-coupled bile acid receptor | EndogenousMetabolite | Endogenous Metabolite | Deoxycholic acid | bile acid |
| Inhibitors Related | Sucrose | Aceglutamide | Nicotinamide riboside malate | DL-Lysine | D(+)-Raffinose pentahydrate | Guanidine hydrochloride | Formamide | Glycerol | Thymidine | Corn starch | Hyaluronic acid sodium (MW 20 kDa) | Gluconate Calcium |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Bioactive Compound Library | Anti-Cancer Clinical Compound Library | Natural Product Library | Microbial Natural Product Library | FDA-Approved Drug Library | Cosmetic Ingredient Compound Library | Bioactive Compounds Library Max | Gut Microbial Metabolite Library | GPCR Compound Library | Anti-Cancer Drug Library | Anti-Cancer Active Compound Library |
United States
