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ChemicalBook Product Catalog Biochemical Engineering Biochemical Reagents Fluorescence Imaging Dihydrorhodamine 123

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Dihydrorhodamine 123 NEW

$33.00 1mg

$79.00 5mg

$112.00 10mg

Min. Order
Purity99.53%
Cas No109244-58-8
Supply Ability10g
Update time2026-04-13

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

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Chemical Properties

Product Name	Dihydrorhodamine 123
CAS No	109244-58-8
EC-No
Min. Order
Purity	99.53%
Supply Ability	10g
Release date	2026/04/13

Product Introduction

Bioactivity

Name	Dihydrorhodamine 123
Description	Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λex=488 nm, λem=525 nm). Dihydrorhodamine 123 is oxidized to the fluorescent reagent Rhodamine 123 in the presence of intracellular reactive oxygen species and is localized in mitochondria.
Cell Research	I. Detection of Reactive Oxygen Species (ROS) 1. Solution preparation: Dissolve DHR 123 in an appropriate solvent (such as DMSO or PBS) at a concentration of typically 1-10 μM. 2. Cell staining: Add DHR 123 solution to cultured cells and incubate at 37°C for typically 30-60 min. 3. Oxidation process: ROS produced in cells oxidize DHR 123, converting it into fluorescent products. 4. Fluorescence measurement: After staining, measure the fluorescence of the oxidation products using a fluorescence spectrophotometer or fluorescence microscope with an excitation wavelength of 488 nm and an emission wavelength of 525 nm. 5. Analysis: Analyze ROS levels in cells by fluorescence intensity. Experiments can be performed in real time or at fixed time points. II. Assessment of mitochondrial function and membrane potential 1. Cell incubation: Incubate cells as described above. 2. Mitochondrial ROS detection: Oxidized DHR 123 will show a fluorescent signal under a fluorescence microscope, indicating the generation of mitochondrial ROS. 3. Fluorescence microscopy observation: Observe the fluorescent signal to see the distribution of ROS in specific areas of the cell (especially mitochondria). III. Flow cytometry for ROS quantitative analysis 1. Cell staining: Add DHR 123 solution to cells as described above. 2. Flow cytometric analysis: After incubation, wash the cells and perform fluorescence analysis using a flow cytometer. The fluorescence intensity is proportional to the ROS level in the sample, which can achieve quantitative analysis. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
In vitro	In the presence of 10 μM Dihydrorhodamine 123 (DHR 123), the stimulation of neutrophil NADPH oxidase with 50 nM phorbol 12-myristate 13-acetate (PMA) increases the rate of rhodamine generation. Similarly, induced HL60 cells exhibit a sustained fluorescence increase after the addition of 50 nM PMA in the presence of 10 μM DHR 123.
Storage	Powder: -20°C for 3 years Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 262.5 mg/mL (757.84 mM), Sonication is recommended.
Keywords	Inhibitor \| inhibit \| Dihydrorhodamine-123 \| Dihydrorhodamine123 \| Dihydrorhodamine 123 \| DHR-123 \| DHR123

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