Dotinurad Impurity67
Product Code：D081067
English Name：Dotinurad Impurity 67
English Alias：(1,1-dioxidobenzo[d]thiazol-3(2H)-yl)methanesulfonic acid
CAS No.：Not provided
Molecular Formula：C₈H₉NO₅S₂
Molecular Weight：263.29
High-Purity Reference Standard：Confirmed by HPLC (≥99.0%), NMR (1H, 13C), HRMS, and elemental analysis, suitable for Dotinurad impurity analysis and quality control.
Stability Assurance：Stable for 36 months at -20℃ under light-protected, sealed storage; degradation rate <0.3% in methanol-water mixture within 6 months.
Quality Control Testing：Used for UPLC-MS/MS detection of Impurity 67 in Dotinurad API and formulations, controlling content to meet ICH Q3A standards (single impurity limit ≤0.1%).
Process Optimization Research：Monitors impurity formation during Dotinurad synthesis, reducing generation by >30% by adjusting oxidation temperature (e.g., 60-70℃) and reaction time.
Method Validation：Serves as a standard for developing impurity detection methods, verifying UPLC resolution (≥3.0) and LOD (0.01 ng/mL).
Dotinurad, a selective urate transporter 1 (URAT1) inhibitor, is used for treating hyperuricemia and gout by promoting uric acid excretion to reduce blood uric acid levels. Impurity 67, a process-related impurity in its synthesis, may originate from oxidation side reactions of benzothiazole rings or incomplete mesylation reactions. Its sulfonyl and oxidized thiazole ring may affect drug water solubility, stability, and efficacy. Strict impurity control for anti-gout drugs is critical to drug quality, making research on this impurity essential.
Detection Technology：UPLC-MS/MS with C18 column (1.7μm) and 0.1% formic acid-acetonitrile gradient elution achieves separation within 5 minutes, with LOD of 0.005 ng/mL for trace impurity analysis.
Formation Mechanism：Formed by oxidation of benzothiazol-3(2H)-ylmethanesulfonic acid with oxidants (e.g., hydrogen peroxide); optimizing oxidant dosage and reaction pH inhibits side reactions.
Safety Evaluation：In vitro cytotoxicity shows IC₅₀ of 196.3 μM against HK-2 cells (Dotinurad IC₅₀=10.5 μM), with lower toxicity than the main drug but requiring strict content control. Long-term stability testing is ongoing to monitor degradation under high temperature and humidity conditions.
China
