| Name | Doxycycline |
| Description | Doxycycline is an orally active tetracycline antibiotic with broad-spectrum inhibitory effects on matrix metalloproteinases (MMPs), possessing both antibacterial and antitumor activities, and is commonly used in inducible gene expression ON-OFF systems. |
| In vitro | METHODS: Twenty-two tumor cells were treated with Doxycycline for 48 h. Cell viability was measured by MTT.
RESULTS: Lung cancer cells were more sensitive to Doxycycline than most other cell lines. NCI-H446 and A549 cells were sensitive to Doxycycline with IC50s of 1.70 μM and 1.06 μM, respectively. [1]
METHODS: Human pancreatic cancer cells PANC-1 were treated with Doxycycline (10-40 μg/mL) for 8-24 h. The cell cycle was detected by Flow Cytometry.
RESULTS: Doxycycline caused the cells to arrest in the G1-S phase of the cell cycle. [2] |
| In vivo | METHODS: To detect anti-tumor activity in vivo, Doxycycline (50 mg/kg) was administered intraperitoneally once daily for fifteen days to BALB/c mice harboring human mammary carcinoma tumor 4T1.
RESULTS: Doxycycline delayed tumor growth and reduced the number of tumor-associated macrophages and blood vessels. [3]
METHODS: To assay antimicrobial activity in vivo, Doxycycline (40 mg/kg) was administered intraperitoneally to BALB/c mice exposed to B. pseudomallei 1026b twice daily for two weeks.
RESULTS: Doxycycline-treated mice survived 100% of the time, and no B. pseudomallei DNA was amplified from the lungs or spleens of the majority of the surviving mice. [4] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : < 1 mg/mL (insoluble)
10% DMSO+90% Saline : 4 mg/mL (9 mM), Solution.
DMSO : 200 mg/mL (450.01 mM), Sonication is recommended.
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| Keywords | U343 | SVG | Parasite | MMP | MCF 12A | Matrix metalloproteinases | LNT-229 | Inhibitor | inhibit | G55 | Doxycycline | Bacterial | anti-proliferative activity | Antibiotic | antibacterial activity | 30S ribosome | 293T | 16S ribosome |
| Inhibitors Related | Neomycin sulfate | Adipic dihydrazide | Levulinic acid | D(+)-Raffinose pentahydrate | Sulfamethoxazole sodium | Terbinafine hydrochloride | Hydroxychloroquine | 2-Amino-2-methyl-1-propanol | Fenpyroximate | Hyaluronic acid sodium (MW 20 kDa) | Dimethyl sulfoxide | Sodium diacetate |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Anti-Parasitic Compound Library | Failed Clinical Trials Compound Library | Bioactive Compound Library | Anti-Cancer Clinical Compound Library | Drug Repurposing Compound Library | Inhibitor Library | FDA-Approved Drug Library | Anti-Cancer Approved Drug Library | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library |
United States
