| Name | Estradiol |
| Description | Estradiol (E2) is a naturally occurring steroidal sex hormone that is essential for female fertility and maintenance of secondary sexual characteristics. Estradiol upregulates IL-6 expression through estrogen receptor beta (ERβ). |
| Cell Research | Estradiol is dissolved in 0.01% ethanol. Briefly, B9 cells (5×103/well of a 96-well plate) are cultured with a series of dilutions of the supernatants in a final volume of 100 mL of RPMI 1640, supplemented with 5×10-5mol/Lof 2-mercaptoethanol, 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin in flat-bottom microtiter plates. After 42 h, 0.5 μCi of [3H]thymidine is added. 6 h later, the cells are harvested and the radioactivity incorporated is determined. IL-6 is quantitated from a standard curve set up with known amounts of recombinant human or mouse IL-6. The anti-IL-6 monoclonal antibody completely inhibited the ability of the B9 cells to proliferate in response to recombinant mouse IL-6. In addition, we determined that B9 cells do not proliferate in response to IL-I or TNF, and that antibodies to these two cytokines did not affect the cell proliferation in response to IL-6. Further, it is established that neither Estradiol (17β-estradiol), nor the 0.01% ethanol used as vehicle in these experiments, has any effect on the bioassay. |
| In vitro | METHODS: Human lens epithelial cells HLEC were treated with Estradiol (0.001-10 μM) for 24 h. Cell viability was measured using the MTT.
RESULTS: The number of viable cells was significantly reduced in the presence of 10 μM Estradiol. [1]
METHODS: Human breast cancer cells MB231 were treated with Estradiol (100 nM) for 24 h. Cell motility was detected using scratch/wound healing assay and transwell assay.
RESULTS: Estradiol treatment reduced the migration and invasion of MB231 cells. [2] |
| In vivo | METHODS: To assay in vivo activity, Estradiol (0.2 mg/kg) and progesterone (5-20 mg/kg) were administered intraperitoneally as a single injection to ovariectomized C57BL/6 female mice.
RESULTS: Mice receiving Estradiol alone or Estradiol plus 10-20 mg/kg progesterone showed significantly enhanced memory for new objects. [3]
METHODS: To assay in vivo activity, Estradiol (0.2 mg/kg) was administered by gavage to ovariectomized C57BL/6 female mice once daily for two weeks.
RESULTS: Long-term activation of ERβ reduced hot flash-like symptoms and enhanced spatial and object recognition memory in ovariectomized mice. [4] |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | H2O : <1 mg/mL (insoluble)
Ethanol : 20.4 mg/mL (74.9 mM), Sonication is recommended.
DMSO : 250 mg/mL (917.84 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2.73 mg/mL (10.02 mM), Suspension.
|
| Keywords | uterine fibroids | steroid sex hormone | progestogenReceptor | progestogen Receptor | neural differentiation | Inhibitor | inhibit | human endometrial stem cells | EstrogenReceptor | Estrogen Receptor/ERR | Estrogen Receptor | Estrogen | Estradiol | ERR | ER | EndogenousMetabolite | Endogenous Metabolite | E-2 | E 2 | beta-Estradiol | b-Estradiol | 17b-Oestradiol | 17beta-Oestradiol | 17beta-Estradiol | 17b-Estradiol |
| Inhibitors Related | Sucrose | Aceglutamide | Nicotinamide riboside malate | DL-Lysine | D(+)-Raffinose pentahydrate | Guanidine hydrochloride | Formamide | Glycerol | Thymidine | Corn starch | Hyaluronic acid sodium (MW 20 kDa) | Gluconate Calcium |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Traditional Chinese Medicine Monomer Library | Bioactive Compound Library | Membrane Protein-targeted Compound Library | Anti-Cancer Clinical Compound Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Drug Repurposing Compound Library | Natural Product Library for HTS | FDA-Approved Drug Library | Anti-Cancer Approved Drug Library | Human Endogenous Metabolite Library | Bioactive Compounds Library Max |
United States
