| Name | Galangin |
| Description | Galangin (Norizalpinin) is an agonist/antagonist of the arylhydrocarbon receptor, and also shows inhibition of CYP1A1 activity. |
| Cell Research | Cells (5.0×103) are seeded and treated with different concentrations of galangin for different periods of time in 96-well plates. The number of viable cells in each well is determined by adding 10 μL of 5 mg/mL MTT solution. Following the 4 hour incubation at 37°C, the cells are dissolved in a 100 μL solution containing 20% SDS and 50% dimethy formamide. The optical densities are quantified at a test wavelength of 570 nm with a reference wavelength of 630 nm using a Varioskan Flash Reader spectrophotometer. |
| In vitro | Through thin-layer chromatography, it has been demonstrated that Galangin impedes the catabolic decomposition of DMBA in a dose-dependent fashion and obstructs the generation of DMBA-DNA adducts, thereby averting DMBA-related suppression of cell proliferation. Additionally, Galangin robustly curtails CYP1A1 enzyme activity in a dose-responsive manner within both intact cells and DMBA-exposed cell-derived microsomes, as ascertained through ethoxyresorufin-O-deethylase activity assays. The inhibition of CYP1A1 by Galangin occurs through a noncompetitive mechanism, as evidenced by double-reciprocal plot analysis. Moreover, Galangin elevates CYP1A1 mRNA levels, suggesting potential aryl hydrocarbon receptor agonism, yet it counteracts the DMBA or TCDD-triggered upregulation of CYP1A1 mRNA and promoter-driven transcription[1]. Furthermore, Galangin administration hampers cell proliferation while propelling autophagy at 130 μM and apoptosis at 370 μM. Specifically, in HepG2 cells, Galangin induces autophagosome accumulation, enhances microtubule-associated protein light chain 3 levels, and augments the proportion of vacuolated cells, alongside a surge in p53 expression. The Galangin-induced autophagic response is mitigated by p53 inhibition in HepG2 cells, while p53 overexpression in Hep3B cells normalizes the elevated vacuole presence[2]. |
| Storage | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | Chloroform, Dichloromethane, Ethyl Acetate, Acetone : Soluble
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (18.5 mM), Sonication is recommended.
DMSO : 237 mg/mL (877 mM), Sonication is recommended.
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| Keywords | NF-κB | NFκB | NF-kB | NFkB | Inhibitor | inhibit | Galangin | ERK | Cytochrome P450 | CYPs | Autophagy |
| Inhibitors Related | Stavudine | Aceglutamide | Hemin | Urea | Tamoxifen | Guanidine hydrochloride | Hydroxychloroquine | Ethyl linoleate | Paeonol | Naringin | Gluconate Calcium | Alginic acid |
| Related Compound Libraries | Polyphenolic Natural Product Library | Pain-Related Compound Library | Selected Plant-Sourced Compound Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Miao medicine Compound Library | Inhibitor Library | Natural Product Library for HTS | Immunology/Inflammation Compound Library | Anti-Aging Compound Library | Anti-virus Traditional Chinese Medicine Monomer Library | Bioactive Compounds Library Max | Heat-Clearing and Detoxifying Traditional Chinese Medicine Compound Library |
United States
