| Name | Glipizide |
| Description | Glipizide (CP 28720) is a short-acting, second-generation sulfonylurea with hypoglycemic activity. |
| Kinase Assay | The activity of caspase-3, -8 and -9 is assessed using the caspase-3, -8 and -9 colorimetric assay kits, respectively. In brief, 1×106?cells in a 60-mm culture dish are incubated with 10 mM Valproic acid for 24 h. The cells are then washed in PBS and suspended in 5 volumes of lysis buffer provided with the kit. Protein concentrations are determined using the Bradford method. Supernatants containing 50 μg total protein are used to determine caspase-3, -8 and -9 activities. The supernatants are added to each well in 96-well microtiter plates with DEVD-pNA, IETD-pNA or LEHD-pNA as caspase-3, -8 and -9 substrates and the plates are incubated at 37°C for 1 h. The optical density of each well is measured at 405 nm using a microplate reader. The activity of caspase-3, -8 and -9 is expressed in arbitrary absorbance units. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 82 mg/mL (184.05 mM), Sonication is recommended.
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
10% DMSO+90% Corn Oil : 3.3 mg/mL (7.41 mM), Sonication is recommended.
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| Keywords | type 2 diabetes | sulfonylurea class | PPARγ | PotassiumChannel | Potassium Channel | pancreatic β | mellitus | KcsA | K-4024 | K4024 | K ATP | Inhibitor | inhibit | Glipizide | CP-28720 | CP28720 | ATP-sensitive potassium | ABCC |
| Inhibitors Related | PHYTOL | Rosiglitazone | Daidzein | Hydrochlorothiazide | 1,8-Cineole | Tetraethylammonium bromide | Maltitol | 2,3-Butanediol | NPC 15199 | Chlorzoxazone | Cloxiquine | Indapamide |
| Related Compound Libraries | Pain-Related Compound Library | Bioactive Compound Library | Anti-Neurodegenerative Disease Compound Library | Membrane Protein-targeted Compound Library | Anti-Cancer Clinical Compound Library | Drug Repurposing Compound Library | FDA-Approved Drug Library | Anti-Cancer Approved Drug Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Ion Channel Targeted Library | Anti-Cancer Drug Library |
United States
