GMP Human IL-2 Protein
Features
1. Designed under ISO 9001:2015 and ISO 13485:2016
2. Manufactured and QC tested under a GMP compliance factory
3. FDA DMF filed
4. Animal-Free materials
5. Beta-lactam materials free
6. Batch-to-batch consistency
7. Stringent quality control tests
Source
GMP Human IL-2 Protein (GMP-L02H14) is expressed from E. coli cells. It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 15.4 kDa. The protein migrates as 16 kDa±2 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
N-terminal Sequence Analysischain1:
chain1:Met-Ala-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys-Thr-Gln-Leu-Gln-Leu
chain2:Ala-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys-Thr-Gln-Leu-Gln-Leu-Glu
(Routinely tested)
Endotoxin
Less than 5.0 EU/Vial, tested by the LAL method in compliance with USP <85> and Ph. Eur. 2.6.14.
Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.
Host Cell DNA
<0.1 ng/μg of protein tested by qPCR.
Sterility
Sterility testing was performed using the membrane filtration method in compliance with USP <71> and Ph. Eur. 2.6.1.
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in phosphate with protectants.
Contact us for customized product form or formulation.
Vial Specification
2R (13 mm neck finish)
Shipping
This product is supplied and shipped with blue ice, please inquire the shipping cost.
Storage
Upon receipt, store it immediately at -20°C or lower for long term storage.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C for 5 years in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
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