| Name | Gramine |
| Description | Gramine (Donaxine), a natural indole alkaloid, present in several plant species, acts as an active adiponectin receptor (AdipoR) agonist, with IC50s of 3.2 and 4.2 μM for AdipoR2 and AdipoR1, respectively. Gramine is also a human and mouse β2-Adrenergic receptor (β2-AR) agonist. Gramine has anti-tumor, anti-viral and anti-inflammatory properties. |
| Kinase Assay | Assay of protein kinase C: Protein kinase C is arrayed by measuring 32PI transferred from [gamma-32PI] ATP to lysine-rich histone type Ill-s. The reaction mixture (80 μL) contained 50 mM Tris-HCI. pH 7.4, 100 μM CaCl2, 10 mM MgCI2, 37.5 μL/mL histone type Ill-s, 10 μM [gamma-32PI] ATP (1250 cpm/pmol), 31 μM bovine brain phosphatidylserine and 0.5 μM 1,2 sn-dioleylglycerol. Fifteen μL of purified PKC (final concentration in assay 0.38 μg/mL) is added to the incubation mixture. After 10 min at 30°C, the reaction is stopped by addition of 30 μL of casein 30 mg/mL and 0.9 ml of 12% trichloroacetic acid. The acid precipitable material is collected by centrifugation, dissolved in 1N NaOH (100μL) and precipitated again with 1 ml of 12% trichloroacetic acid. The pellet is dissolved in 1N NaOH (100μL) and 32P incorporation is measured by scintillation counting in Aquasol. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | Ethanol : 33 mg/mL (189.39 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble or slightly soluble)
2% DMSO+30% PEG300+5% Tween 80+63% Saline : 2.4 mg/mL (13.77 mM)
DMSO : 120 mg/mL (688.71 mM), Sonication is recommended.
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| Keywords | ReverseTranscriptase | Reverse Transcriptase | NSC-16892 | NSC16892 | Inhibitor | inhibit | Gramine | Beta Receptor | AdrenergicReceptor | Adrenergic Receptor | AdipoRs | Adiponectinreceptor | Adiponectin Receptor |
| Inhibitors Related | Stavudine | Emtricitabine | Lamivudine | Octopamine hydrochloride | Tenofovir Disoproxil Fumarate | Dexmedetomidine hydrochloride | Isoprenaline hydrochloride | D-Mannitol | Mianserin hydrochloride | Trazodone hydrochloride | Tenofovir | Doxepin hydrochloride |
| Related Compound Libraries | Bioactive Compound Library | Traditional Chinese Medicine Monomer Library | Membrane Protein-targeted Compound Library | Selected Plant-Sourced Compound Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Natural Product Library for HTS | Immunology/Inflammation Compound Library | Anti-Aging Compound Library | Anti-infective Natural Product Library | Bioactive Compounds Library Max | GPCR Compound Library | Food as Medicine Compound Library |
United States
