| Name | GW 4064 |
| Description | GW 4064 is an effective farnesoid X receptor (FXR) agonist (EC50 =65 nM). |
| Cell Research | GW 4064 is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. Mouse liver cells (BNL CL.2) are maintained in a humidified incubator under 5% CO2 at 37°C in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin. When cells are divided into six-well plates and reach ~90% confluence, sub-confluent cells are washed three times with phosphate buffered saline (PBS) and replaced with serum-free DMEM supplemented with 1% fatty acid-free BSA. Oleic acid (final concentration 500 μM) and GW4064 at various concentrations are added and incubated for 24 h. Cells are then fixed with 4% formaldehyde for Oil Red O staining or harvested for protein and western blot analysis[2]. |
| In vitro | In Fisher rats, oral administration of GW 4064 (ED50 = 20 mg/kg) effectively reduces serum triglycerides. |
| In vivo | In CV-1 cells transfected with mouse FXR expression vectors (EC50=80 nM) and in CV-1 cells transfected with human FXR expression vectors (EC50=90 nM), GW 4064 functions as a selective non-steroidal FXR agonist, effectively exerting agonistic effects. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 50 mg/mL (92.11 mM), Sonication is recommended.
Ethanol : 5.4 mg/mL (9.95 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (3.68 mM), Sonication is recommended.
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| Keywords | NR1H4 | Inhibitor | inhibit | GW-4064 | GW4064 | GW 4064 | FXR | Autophagy |
| Inhibitors Related | Stavudine | Aceglutamide | Hemin | Tamoxifen | Cysteamine hydrochloride | Guanidine hydrochloride | Hydroxychloroquine | Enzalutamide | Paeonol | Naringin | Alginic acid | Sildenafil citrate |
| Related Compound Libraries | Nuclear Receptor Compound Library | Ferroptosis Compound Library | Bioactive Compound Library | ReFRAME Related Library | Autophagy Compound Library | NO PAINS Compound Library | Metabolism Compound Library | Lipid Metabolism Compound Library | Bioactive Compounds Library Max | Anti-Metabolism Disease Compound Library |
United States
