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ChemicalBook Product Catalog HADA Hydrochloride

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HADA Hydrochloride NEW

$66.00 1mg

$158.00 5mg

$248.00 10mg

Min. Order
Purity98.51%
Cas No2253733-10-5
Supply Ability10g
Update time2026-04-22

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

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United States

United States

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Chemical Properties

Product Name	HADA Hydrochloride
CAS No	2253733-10-5
EC-No
Min. Order
Purity	98.51%
Supply Ability	10g
Release date	2026/04/22

Product Introduction

Bioactivity

Name	HADA Hydrochloride
Description	HADA Hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λem~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis. HADA Hydrochloride can be used in specific and covalent probing of bacterial growth with minimal perturbation.
Cell Research	HADA labeled PG site experiment of E. coli cells I. Solution preparation Mother solution preparation: Prepare 50 mM (100×) stock solution with DMSO; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) II. Operation steps 1. Inoculate cells from -80°C bacterial cryopreservation solution into sterile liquid culture medium and culture cells using optimal species-specific conditions until exponential growth is achieved. 2. Add 10 μL of 50 mM HADA DMSO stock solution to the culture tube. 3. Dilute the culture to OD600 = 0.05 in fresh LB; 4. Add 990 μL of the culture to the culture tube containing FDA to obtain a final LB solution of [HADA] = 500 μM and [DMSO] = 1%, and incubate the culture at 37°C with shaking; 5. When the culture reaches OD600 = 0.4 – 0.6, pellet the cells (1 min, maximum speed in a microcentrifuge, room temperature); 6. Resuspend the cells in 1 mL 70% (vol/vol) ice-cold EtOH and fix the cells on ice for 10 – 15 min. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
In vitro	FDAA labeling can be completed in as little as 30 seconds for rapidly growing species like Escherichia coli. Despite HADA Hydrochloride being dimmer and less photostable compared to FDL or TDL, it consistently and effectively labels the PG of a wide range of bacterial species, usually without requiring significant optimization[1].
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 112.5 mg/mL (342.25 mM), Sonication is recommended.
Keywords	HCC-Amino-D-alanine Hydrochloride \| HADA Hydrochloride
Inhibitors Related	Neomycin sulfate \| Aceglutamide \| Hemin \| D(+)-Raffinose pentahydrate \| Guanidine hydrochloride \| Sulfamethoxazole sodium \| Terbinafine hydrochloride \| Hydroxychloroquine \| Hyaluronic acid sodium (MW 20 kDa) \| Dimethyl sulfoxide \| Sodium diacetate \| Sodium bicarbonate
Related Compound Libraries	Bioactive Compound Library \| Bioactive Compounds Library Max

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