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ChemicalBook Product Catalog Hoechst 33258 trihydrochloride

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Hoechst 33258 trihydrochloride NEW

$ 53.00 /50mg

Min. Order
Purity99.47%
Cas No23491-45-4
Supply Ability10g
Update time2026-04-14

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

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United States

United States

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Chemical Properties

Product Name	Hoechst 33258 trihydrochloride
CAS No	23491-45-4
EC-No
Min. Order
Purity	99.47%
Supply Ability	10g
Release date	2026/04/14

Product Introduction

Bioactivity

Name	Hoechst 33258 trihydrochloride
Description	Hoechst 33258 trihydrochloride (Bisbenzimide) is a benzimidazole anti-filarial agent. it is fluorescent when it binds to certain nucleotides in DNA, thus providing a tool for the study of DNA replication. it also interferes with mitosis.
Cell Research	Solution preparation a. Preparation of stock solution: Dissolve 10 mg in 5 mL DMSO; (It is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) b. Preparation of Hoechst working solution Dilute the stock solution and dissolve it in serum-free cell culture medium or PBS to obtain a Hoechst working solution with a final concentration of 10 μg/mL; (Select the appropriate working solution concentration according to experimental requirements and prepare it for immediate use) c. Cell staining 1. Suspended cells (6-well plate) a. Centrifuge at 1000 g for 3-5 minutes at 4℃, and then discard the supernatant. Wash twice with PBS for 5 minutes each. The cell density is 1×106/mL; b. Add 1 mL of working solution and incubate at room temperature for 3-10 minutes; c. Centrifuge at 400 g for 3-4 minutes at 4℃, and discard the supernatant; d. Wash twice with PBS, 5 minutes each time; e. Resuspend cells with serum-free cell culture medium or PBS. Observe with fluorescence microscope or flow cytometer. 2. Adherent cells a. Culture adherent cells on sterile coverslips; b. Remove coverslips from culture medium and aspirate excess culture medium; c. Add 100 μL working solution, shake gently to completely cover cells, and incubate at room temperature for 3-10 minutes; d. Wash twice with culture medium, 5 minutes each time. Observe with fluorescence microscope or flow cytometer. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Storage	Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 6.58 mg/mL (12.32 mM), Sonication is recommended.
Keywords	Parasite \| Inhibitor \| inhibit \| Hoechst 33258 trihydrochloride \| HOE-33258 \| HOE33258 \| H-33258 Trihydrochloride \| H33258 Trihydrochloride \| H-33258 \| H33258 \| H 33258 Trihydrochloride \| H 33258 \| bisBenzimide H-33258 Trihydrochloride \| bisBenzimide H33258 Trihydrochloride \| bisBenzimide H-33258 \| bisBenzimide H33258 \| bisBenzimide H 33258 Trihydrochloride \| bisBenzimide H 33258
Inhibitors Related	Flubendazole \| Gum arabic \| Kojic acid \| Urethane \| Hydroxychloroquine \| Metronidazole \| Avermectin B1a \| 2-Amino-2-methyl-1-propanol \| Doxycycline \| Fenpyroximate \| Methylene Blue trihydrate \| Coumaran
Related Compound Libraries	Anti-Parasitic Compound Library \| Bioactive Compound Library \| NO PAINS Compound Library \| Bioactive Compounds Library Max \| Anti-Infection Compound Library \| Human Metabolite Library

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