Source
HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
Clone
6F11C8
Species
Mouse
Isotype
Mouse IgG1 | Mouse Kappa
Conjugate
HRP
Antibody Type
Hybridoma Monoclonal
Reactivity
Human
Immunogen
Human-IgG-Fc
Specificity
This product can react with the Fc portion of Human IgG1/ IgG2/ IgG3/ IgG4 and multiple engineered variants, including: Human IgG1 (N297A), Human IgG1 (L234A/L235A), Human IgG1 (M252Y/S254T/T256E), Human IgG1 (L234F/L235E/P331S), Human IgG1 (S239D/I332E), Human IgG1 (S298A/E333A/K334A), Human IgG1 (T307A/E380A/N434A), Human IgG2 (A330S/P331S), Human IgG2 (P331S), Human IgG4 (S108P), and Human IgG4 (S228P/L235E).
Application
Application | Recommended Usage |
ELISA | 0.7-200 ng/mL |
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 6 months after reconstitution;
2-8°C for 2-3 weeks under sterile conditions after reconstitution.
Cross Verification
This antibody recognizes all four human IgG isotypes (IgG1, IgG2, IgG3, and IgG4) and multiple engineered variants, including: Human IgG1 (N297A), Human IgG1 (L234A/L235A), Human IgG1 (M252Y/S254T/T256E), Human IgG1 (L234F/L235E/P331S), Human IgG1 (S239D/I332E), Human IgG1 (S298A/E333A/K334A), Human IgG1 (T307A/E380A/N434A), Human IgG2 (A330S/P331S), Human IgG2 (P331S), Human IgG4 (S108P), and Human IgG4 (S228P/L235E).
Weak cross-reactivity with cynomolgus IgG was observed by ELISA, whereas no reactivity to mouse IgG isotypes (IgG1, IgG2a, IgG2b) was detected.
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