Synonym
CD3 epsilon & CD3 gamma, CD3E & CD3G
Source
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (CDG-H52W5) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).
Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
Request for sequence
Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.9 kDa (CD3E) and 14.6 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 19 kDa, 21 kDa and 26-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
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