Biotinylated Human IGFBP-5 Protein, Avitag™,Flag&His Tag (MALS verified)
Synonym
IBP5, IGFBP5
Source
Biotinylated Human IGFBP-5 Protein, Avitag,Flag&His Tag (IG5-H82D3) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - Glu 272 (Accession # P24593).
Predicted N-terminus: Gly
Molecular Characterization
This protein carries an Avi tag (Avitag™) at the N-terminus followed a Flag tag and at the C-terminus by a polyhistidine tag. The protein has a calculated MW of 33.3 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Background
IGFBP5 stands for Insulin-like Growth Factor Binding Protein 5. It is a protein that belongs to the insulin-like growth factor binding protein (IGFBP) family. IGFBP5 plays a crucial role in regulating the activity and distribution of insulin-like growth factors (IGFs) in the body. Enables insulin-like growth factor I binding activity.
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