Human IL-20 Protein, His Tag (MALS & SPR verified)
Synonym
interleukin-20, IL10D, IL-20, interleukin 20, Cytokine Zcyto10, ZCYTO10
Source
Human IL-20, His Tag (IL0-H5243) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Glu 176 (Accession # Q9NYY1-1).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 19.4 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Background
Interleukin-20 (IL-20) belongs to the family of IL-10-related cytokines, which includes IL-10, -19, -20, -22, -24 and -26. IL-20 induces STAT activation through two types of receptor complex, one of which it shares with IL-19, indicating that their biological activities may overlap. Both cytokines may play an important role in regulating the development and proper functioning of skin. IL-20 has also been shown to promote endothelial cell tube formation, suggesting that it may be involved in vascular vessel maturation and remodelling.
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