Human Siglec-7 / CD328 Protein, His Tag (MALS verified)
Synonym
CDw328, D-siglec, A79 membrane protein, p75, Adhesion inhibitory receptor molecule 1, AIRM-1
Source
Human Sigelc-7, His Tag (SG7-H5225) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).
Predicted N-terminus: Gln 19
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 38.8 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Background
Siglec-7 is a member of the human CD33‐related Siglec receptor. The extracellular region of Siglec‐7 is characterized by an N‐terminal V‐set Ig domain that can bind sialic acid and two C2‐set Ig domains. The cytoplasmic tail of Siglec‐7 has one immune‐receptor tyrosine‐based inhibitory motif (ITIM) and one ITIM‐like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.
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